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VERSION:2.0
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CALSCALE:GREGORIAN
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DTSTAMP:20260728T030654Z
UID:46a364c5-4063-406e-bba6-f61427f54fb8
DTSTART:20241209T080000Z
DTEND:20241213T120000Z
DESCRIPTION:Educators:\nRobert Heyer\, Dirk Benndorf\, Patrick Hellwig\, Ka
 y Schallert\, Daniel Kautzner\, Claudia Best (BiGi/MetaProtServ)\n\nDate:\
 n09.12.2024 – 13.12.2024\n\nLocation:\nMagdeburg\, Germany (Universität
 splatz 2\, Building 28.217)\n\nContents:\nThe field of microbiome research
  is experiencing significant growth\, with Meta-Omics data offering valuab
 le insights into microbial communities that impact human health and bioche
 mical cycles. Metaproteomics\, a branch of Meta-Omics\, focuses on analyzi
 ng proteins to identify expressed genes and their corresponding functions.
  Unlike metagenomics or metatranscriptomics\, metaproteomics requires the 
 extraction and analysis of proteins from human or environmental samples\, 
 a complex and challenging process.\n\nThe de.NBI course on Applied Metapro
 teomics is designed to train participants in the complete metaproteomics w
 orkflow\, from experimental design and sample preparation to measurement a
 nd bioinformatics analysis of high-resolution mass spectrometry (MS) data.
  The course accommodates a maximum of 10 participants\, who are encouraged
  to bring their own samples to begin their metaproteomics projects.\n\nThe
  workshop will be held at the Otto-von-Guericke University Magdeburg by Pr
 of. Dirk Benndorf and Prof. Robert Heyer. Participants will have the oppor
 tunity to work with leading experts in the field\, gaining practical exper
 ience and expert guidance throughout the course.\n\nMonday\n09:00-10:00   
 Arrival\n10:00-11:00   Introduction to metaproteomics (lecture: Dirk Bennd
 orf)\n11:00-12:00   Design of experiments and sample preparation (lecture:
  Robert Heyer)\n12:00-13:00   Lunch\n13:00-13:30   Safety instructions (Cl
 audia Best)\n13:30-18:00   Sample preparation I (protein extraction\, prot
 ein quantification: Claudia Best\, Backup-Max)\n\nTuesday\n08:00-11:00   S
 ample preparation II (FASP digest\, SDS-PAGE: Claudia Best)\n11:00-13:00  
  Introduction to high resolution mass spectrometry (lecture: Dirk Benndorf
 )\n13:00-14:00   Lunch\n13:00-14:00   Sample preparation II (peptide extra
 ction: Claudia Best)\n14:00-16:00   Introduction to the MPA 2.0 (lecture: 
 Kay Schallert)\n18:00-20.00   Sightseeing in Magdeburg\nnon-stop: measurem
 ent of participant’s samples I\n\nWednesday\nnon-stop: measurement of pa
 rticipant’s samples I: (Dirk Benndorf\, Patrick Hellwig)\n09:00-11:00   
 MPA 2.0 exercise I (working with a small reference dataset\, db searching\
 , metaprotein concept: Kay Schallert\, Robert Heyer)\n11:00-13:00   MPA 2.
 0 exercise II (loading user db\, BLAST search of unknown hits\, quantitati
 ve comparison of datasets\, visualization and data export: Kay Schallert\,
  Robert Heyer)\n13:00-14:00   Lunch\n14:00-17:00   Training at MS (calibra
 tion\, setup of LC-MS methods\, solvent exchange\, trouble shooting\, Jan)
 \n\nThursday\n09:00-13:00   MPA 2.0 exercise III (analyzing own data: Kay 
 Schallert)\n13:00-14:00   Lunch\n14:00-15:00    TBA (lecture and training:
  NN) \n15:00-17:00    Metaproteomics Initiative and NFDI Standardization I
  (Kay Schallert)\n18:30-20:00   Dinner (Restaurant “Lago di Garda”)\n\
 nFriday\n08:00-10:00   MPA 2.0 exercise IV (visualization of data: Robert 
 Heyer)\n10:00-12:00   Individual discussion of results and preparation of 
 presentations (Dirk Benndorf\, Robert Heyer\, Kay Schallert)\n12:00-13:00 
   Metaproteomics Initiative and NFDI Standardization II (Kay Schallert)\n1
 3:00   Lunch and departure\n\nLearning goals:\nTo apply a complete workflo
 w starting from design of experiments via sample preparation to measuremen
 t and bioinformatics analysis of high-resolution MS data.\n\nPrerequisites
 :\n- Fee: 100 €\n- Οwn Laptop\n- Own samples could be analyzed while wo
 rkshop (but we could also provide samples) \n\nKeywords:\nMicrobiome\, MPA
 \, Metaproteomics\, MetaProteomeAnalyzer\,\n\nTools:\nMetaproteomeAnalyzer
LOCATION:Magdeburg
SUMMARY:Applied Metaproteomics Workshop Magdeburg 2024
URL;VALUE=URI:https://www.denbi.de/training-courses-2024/1729-applied-metap
 roteomics-workshop-magdeburg-2024
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