e-learning
Mapping
Abstract
Sequencing produces a collection of sequences without genomic context. We do not know to which part of the genome the sequences correspond to. Mapping the reads of an experiment to a reference genome is a key step in modern genomic data analysis. With the mapping the reads are assigned to a specific location in the genome and insights like the expression level of genes can be gained.
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- What is mapping?
- What two things are crucial for a correct mapping?
- What is BAM?
Learning Objectives
- Run a tool to map reads to a reference genome
- Explain what is a BAM file and what it contains
- Use genome browser to understand your data
Licence: Creative Commons Attribution 4.0 International
Keywords: Sequence analysis, deutsch, español, italiano
Competency level: • Beginner
Target audience: Students
Resource type: e-learning
Version: 46
Status: Active
Prerequisites:
- Introduction to Galaxy Analyses
- Quality Control
Learning objectives:
- Run a tool to map reads to a reference genome
- Explain what is a BAM file and what it contains
- Use genome browser to understand your data
Date modified: 2026-03-30
Date published: 2016-10-04
Authors: Joachim Wolff, 
Bérénice Batut, Helena Rasche
Contributors: Ekaterina Polkh, Mehmet Tekman, Niall Beard, William Durand, Anthony Bretaudeau, Björn Grüning, Bérénice Batut, Helena Rasche, Maria Doyle, Nicola Soranzo, Saskia Hiltemann, Teresa Müller, Tristan Reynolds
Scientific topics: Sequence analysis, Mapping
Activity log
